The report explores the advances and lessons learned from Argentina, Chile, Colombia, El Salvador and Peru as regards seed capital for financing new enterprises with high growth potential. After performing an inventory of the public and private financial instruments to support fast growing businesses, the study focuses on four cases of Chile and Colombia which, because of their antiquity and variety, offer an interesting learning opportunity to the people in charge of designing this sort of interventions. These cases are: the subsidy lines to innovative ventures and the lines for cofinancing risk capital funds of the Production Fostering Corporation of Chile, the angel investors’ net of the Octantis incubator in Chile and the risk capital fund Mercurius Ventures in Colombia. The opinions expressed herein are those of the author(s) and do not necessarily represent the official position of the Inter-American Development Bank Permission is granted to reproduce this paper in whole or in part for noncommercial purposes only and with proper attribution to the author(s), the Sustainable Development Department, and the Inter-American Development Bank. Available in Spanish only.